Petri film Plates Preparation Instructions

• Sample Preparation.

• Aerobic Count Plate (A/C)

• E. Coli/Coli form Count Plate (EC)

• Rapid S. Aureus Count Plate (RSA)

Sample Preparation

Cut Sample to small pieces	Put them into plastic bag and weighted
Fill 90 ml distil water in the sample bag	Blend on chopping board with hammer

Top^

Aerobic Count Plate (A/C)

Inoculation With pipette perpendicular to AC, place 1 ml of sample onto center of bottom film	Inoculation Gently apply pressure on spreader to distribute inoculums over circular area before gel is formed. Do not twist of slide spreader.
Incubation Incubate plates with clear slide up in stacks of no more than 20.	Interpretation After 24 hour, AC can count with illuminated magnifier.

Top^

E. Coli/Coli form Count Plate (EC)

Inoculation With pipette perpendicular to EC, place 1 ml of sample onto center of bottom film	Inoculation Gently apply pressure on spreader to distribute inoculums over circular area before gel is formed. Do not twist of slide spreader.
Incubation Incubate plates with clear slide up in stacks of no more than 20.	Interpretation After 24 hour, Coli forms can count and after 48 hours EC can count with illuminated magnifier.

Top^

Rapid S. Aureus Count Plate (RSA)

Inoculation With pipette perpendicular to RSA, place 1 ml of sample onto center of bottom film.	Inoculation Gently apply pressure on spreader to distribute inoculums over circular area before gel is formed. Do not twist of slide spreader.